Sentence examples for shock buffer from inspiring English sources

For the total leaf protein extraction, the leaf disks of equal size were cut from mature leaves and carefully homogenized in grinding buffer, composed of shock buffer (10 mM HEPES-KOH, pH 7.6, 5 mM sorbitol, 5 mM MgCl2 and 10 mM NaF) and solubilization buffer [ 36] (1:1, v:v).

For protein purification, periplasmic space content was recovered by adding 50 mL of osmotic shock buffer (30 mM TrisHCl, pH 7.2, 40% saccharose, 2 mM EDTA) to 2.5 g of cell paste (from 1 L of fermentation broth) and incubating for 10 min at room temperature; cells were collected by centrifugation at 17,200  g for 20 min and the pellet was quickly dissolved in 45 mL of cold 1 mM MgCl2.

The cells from 5 ml of culture were incubated with 0.5 ml of 0.5 M Tris/HCl (pH 7.8) for 10 min at room temperature, then harvested by centrifugation at 2773  g for 10 min at 4°C. Cell pellets were washed once with 5 ml of LB buffer then resuspended in 1 ml of ice-cold osmotic shock buffer [30 mM Tris/HCl, pH 7.8, 40% (w/v) sucrose and 2 mM EDTA].

In order to avoid such large impact forces, anti-shock buffers might be adopted but good buffer design requires knowledge of the impact forces.

A third explanation would be that specific phage types rupture due to osmotic shock during buffer exchange releasing their genome.

The spheroplasts were washed two more times and then lysed by osmotic shock in lysis buffer containing 50 mM Tris pH 8.0, 10 mM MgSO4, 1 mM EDTA, 10 mM potassium acetate, and 1 mM DTT with a cocktail of protease inhibitors.

Between 4 and 8 T75 flasks of MMLV infected B3T3 were collected and lysed using hypotonic shock in homogenisation buffer A followed by mechanical disruption by passing the cell suspension through a 23-G needle six times.

And it is not flexible enough to deal with economic shocks without the buffer of an independent exchange rate and monetary policy.

The analysis explores local responses to climate shocks, food security and poverty reduction, through informal institutions, forms of livelihood diversification and collective land-use systems that allow reciprocity, flexibility and the ability to buffer shocks.

Pellets of cultured cells were lysed in SDS-PAGE-buffer, shock frozen in liquid nitrogen and heated for 5 minutes at 95°C.

This is of no surprise because Hsp70 and other heat shock proteins are "buffers" of cellular stress, and thus such manipulations that lead to proteome stress may impose artificial interactions of Hsp70s with the transfected protein.