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Plasma was separated by centrifugation (1,000g) at 4ºC within half an hour, put on ice until completion of the experiment, and then stored at −30°C until shipment for analysis.
Plasma was collected and frozen (−68 to −78°C) until shipment for analysis.
All samples were stored at −20°C or colder until shipment for analysis at Worldwide Bioanalysis Group, Ware, UK.
Amniotic fluid samples were also collected from each dam, pooled by litter, and frozen (< −20°C) until shipment for analysis.
UK samples were transported frozen on dry ice while samples collected from Lahore Children's Hospital, Pakistan, were lyophilised (freeze dried) prior to shipment for analysis in the UK.
Fetuses were removed from the uterus, and whole brain tissues were removed, individually snap frozen in liquid nitrogen, and maintained frozen (< −68°C) until shipment for analysis.
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The samples were stored in a freezer at -80°C for a maximum of 10 months at Tartu University Hospital, Estonia and were sent to Tampere University Hospital in a single shipment for further analysis.
Water samples were stored at room temperature until shipment to Columbia University for analysis.
They were then blindly returned with every shipment of samples for analysis to assess storage, transport, and laboratory preparation effects.
Immediately after collection, the urine samples were transported to the CCCEH laboratory, inventoried and frozen at –80°C before shipment to CDC for analysis.
"The E.P.A. uses vermiculite to pack samples for shipments to laboratories for analysis," the Grace executive said.
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