Sentence examples for shifts were sequenced from inspiring English sources
Any fragments showing migration shifts were sequenced directly from the PCR products using the ABI Ready Reaction Dye Terminator Cycle Sequencing Kit and an ABI3100 Prism sequencer and analysed using the Sequence Navigator software.
TP53 gene mutation analysis was performed for exons 5 8 (the DNA binding domains) by the PCR-single strand conformation analysis (SSCA) technique and samples displaying mobility shifts were sequenced with the dideoxy termination method to confirm the nucleotide changes.
All cell lines and primary samples were analyzed for TP53 gene status (exons 2 11) by PCR amplification followed by single-strand conformation analysis (SSCA) according to the original protocol and samples displaying gel mobility shifts were sequenced to confirm the nucleotide change.
Genomic DNA from cases showing mobility shifts was sequenced using the BigDyeTerminator Cycle Sequencingkit and a 3730xl automated sequencer (Applied Biosystems, Foster City, USA).
However, variants of samples without the polymorphism but with a shift were sequenced to find the cause of the shift.
The 400 bp PCR fragments formed heteroduplexes for analysis on the Transgenomic WAVE Machine (Transgenomic, Inc., Omaha, NE) and samples with shifted peaks were sequenced to confirm and characterize the variant [ Brightwell et al., 2002].
Samples showing a shift in mobility were sequenced as described above.
All the coding regions including the intron exon boundaries and the promoter (to c.-298) of the LDLR, exon 2 to 12 of PCSK9 and part of exon 26 of APOB including p.R3527, were screened by HRM and those samples with a shift in melting temperature were sequenced.
Recent shifts in how road projects are sequenced have escalated the risk that the government will unnecessarily take homes and other private properties, attorney Andrew Brigham said.
Any fragments showing migration shifts were reamplified and sequenced directly using the ABI Prism dRhodamine Terminator Cycle Sequencing Ready Reaction Kit and an ABI377 Genetic Analyser.
Samples showing different migration shifts were chosen for sequencing.
