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The conformational space available to disordered Nup153FGPxFG was sampled using the Flexible-meccano statistical coil description (Ozenne et al., 2012) and representative ensembles in agreement with experimental chemical shifts were selected using ASTEROIDS (Jensen et al., 2010) and the ensemble was subsequently cross-validated against experimental RDCs and SAXS.
Frequency-specific C chemical shifts were selected prior to C{F} REDOR experiments using rotor-asynchronous Dante irradiation, z-axis storage, and mixing times between 200 and 1200 ms with no H decoupling. Typically, spectra from 100-mg cell-wall samples were the result of the accumulation of 16 384 scans at room temperature.
These chemical shifts were selected for relative quantification (based on signal integration normalized to internal standard) of these metabolites during ripening as shown in Figure 2. A wide range of metabolites is present which includes amino and organic acids (resonances observed in the region of δ 0.80 to 4.00) together with sugars (δ 4.00 to 5.50) and phenolic compounds (δ 5.50 to 8.50).
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The power shifts are selected based on their potential to simultaneously reduce overloads and prevent new ones.
Second, it seems probable--and perfectly logical--that insect groups that are chosen for phylogenetic studies on host-plant shifts are selected preferentially from taxa in which species are known a priori to be relatively specialized and to exhibit clear interspecific variation in host-plant use (e.g., [ 19, 40, 61]).
In fact, at first a shift is selected by using the shift specification algorithm.
If the shift is selected properly, the proposed method can give very satisfactory results after only one iteration.
To test the false-positive rate of our LoF variants, 34 SNPs (15 stop gain and 19 splice site variants) and 24 frame-shift indels were selected for genotyping.
For each experiment, the mRNA decay rate was determined immediately prior to heat shock and at two different times after the shift; these times were selected based on decay kinetics according to the kD values predicted by the mathematical algorithm for each particular gene.
Three shifts from each hospital were selected.
Only day shifts during the week were selected because only then physicians are clearly assigned to a clinical unit (which is important for question 1b); in all other type of shifts physicians are required to work across departments and wards.
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