Sentence examples for shifting complex from inspiring English sources

Transmeta's Crusoe chips, which take their name from the fictional character Robinson Crusoe and are intended for portable computing applications, are intended to drastically simplify computer hardware design by shifting complex operations to software that can be easily modified and quickly updated.

Electrophoretic mobility shift assay (EMSA) using the 5'UTR as a probe and HeLa nuclear extract showed a single shifted complex (Fig. 1B).

As demonstrated in Fig. 3B, while addition of anti-GFP antibody did result in a supershifted complex (lane 3, designated "SS"), addition of a nonspecific control anti-polyhistidine antibody did not alter the mobility of the shifted complex (lane 4).

Nuclear extract from curcumin-treated cells formed a stronger shifted complex designated as Cm.

Although larger area was covered by the shifted complex in EMSA by nuclear extracts from Nonabokra and Pokkali, autoradiogram prepared from shorter exposure did not show multiple complexes or any qualitative difference within shifted complex (data not shown).

EMSA with S80 alone did not show any shifted complex, thus nullifying the first probability (data not shown).

An α-enolase polyclonal antibody was able to bind and supershift the DNA-protein complex and could be effectively competed with excess MP2 but not BEE-1, further demonstrating specificity of the shifted complex (Fig. 9B).

We were able to demonstrate that Sp1 bound this sequence, as competition with excess cold Sp1 consensus sequence and addition of Sp1-specific antibody (αSP1) resulted in the abrogation of a shifted complex (Fig. 4).

Dissociation constants (Kd) were derived from data point fitting with Prism (Graphpad) according to the function for specific binding with hill slope: B = Bmax*[X]h/ Kd h + [X] h ) – where [X] is the concentration of protein X, h is the Hill coefficient, B is the fraction of shifted complex, and Bmax is the maximal amount of complex formed.

Briefly, 10 µl of cervicovaginal specimens were mixed with radiolabeled hemicatenated DNA (hcDNA) and HMGB1-hcDNA shifted complexes were analyzed by electrophoresis on nondenaturing polyacrylamide gel.

The proteins in the shifted complexes were identified by transferring the gels to a membrane, followed by western blot analysis using PUF60 and U2AF65 antibodies [29](Figure S6).