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The enrichment analysis of DMRs with AD-associated eQTLs was conducted using the GoShifter package (https://www.broadinstitute.org/mpg/goshifter/). GoShifter estimates the significance of overlap between trait-associated variants (AD-associated eQTLs) and epigenome annotations (DMRs), by generating null distributions of randomly shifting annotations locally within a tested region.
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Both read counts can be obtained by submitting mapped sequence reads to HTSeq twice with coding gene annotation (e.g., gene.gtf) and shifted gene annotation (shift-gene.gtf) as discussed in Methods Section.
For example, our previous work indicated that shifting curators' attention from annotation to ontology development was a major bottleneck in the annotation workflow (5), due to the associated switch of context and software tools.
This shifts the provenance annotations from the URI syntax, where they cannot be used by semantic engines, to a properly RDF formalized set of provenance statements.
The correction of the previously short-called homopolymer reads through our sequencing efforts gave a fully annotated finished sequence of C. autoethanogenum without the erroneous frame-shift containing annotations which had occurred previously.
The key point of this paper is that by going from a small genome like D. melanogaster to a bigger genome like C. capitata we shift the comparative annotation problem from one we do not yet know how to solve to one that we do.
But there has to be an equal consistency of style among the cast members (who here include no less a star than Kevin Kline in a supporting role) — the feeling that they are all working toward the same end, O comrades of the arts, as they step in and out of the action, shifting among dialogue, monologue, songs and authorial annotation.
We will not report the detailed annotations of these 616 phrases in this paper because the detected polarity shifting patterns in these phrases were both low frequency and long.
Shifting currents.
Perspective shifting.
Shifting cultivation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com