Your English writing platform
Discover LudwigSuggestions(1)
Exact(3)
Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av).
We have used blue shifted variants of green fluorescent protein (cyan fluorescent protein or CFP), orange variant of Discosoma coral (DsRed) and yellow mutant of Zoanthus sp. (zFP) chromophore fragments as spin couplers.
The most commonly employed fluorescent proteins in FRET studies are the spectrally shifted variants of green fluorescent protein, CFP (cyan) and YFP (yellow) [23].
Similar(57)
Plates were incubated for 14 h at 37°C prior to picking individual colonies (in the case of site-directed mutagenesis for creation of blue shifted variants) or fluorescence-based library screening for red shifted variants.
While proton-pumping activity was disrupted in many of the spectrally shifted variants, we identified single tuning mutations that incurred blue and red shifts of 42 nm and 22 nm, respectively, that did not disrupt proton pumping.
Furthermore, vaccines might provide immunological pressure on the circulating strains, which might engender the emergence of drifted or shifted variants with enhanced potential for pathogenicity in humans [1].
This suggests that the shifted variant is either a result of read errors or a true 5' isomiR-the likes of which have been demonstrated in multiple species [ 47].
In the future, new probes under development based on red-shifted variants of GFP-aequorin may improve the sensitivity to undertake imaging of the brain and other deep tissues, like the liver and the heart [36].
Genetically encoded reporters have been produced using the cAMP-binding domains of Protein Kinase A [2], [3] or Epac [4], fused to spectrum-shifted variants of Green Fluorescent Protein (GFP).
Red-shifted variants of the fluorogenic phosphine have been synthesized, but these compounds undergo rapid nonspecific phosphine oxidation.
However, the focus of ongoing research has shifted towards genomic variants of phase I and II drug metabolizing enzymes with one important goal being an individual dose adjustment according to a patient's genotype.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com