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Recall in the phase shifted group at 24 hr post-training was greater than the non-shifted group at 18 hr post-training (t7 = 3.99, P = 0.01), but otherwise, recall exhibited by the phase-shifted groups did not exceed that of the non-shifted groups at other testing times.
In contrast, the stress (foot shock) evoked responses were significantly larger in the phase shifted group.
The phase shifted group performed considerably worse than the control group, demonstrating reduced fear conditioned freezing when placed in the same context (Fig. 2C).
Post-hoc Bonferroni's t-tests revealed significantly reduced freezing in the phase shifted group on all 7 days of testing (Fig. 2C).
Within the shifted group, the recall of contextual fear conditioning peaked in the group tested at 24 hr post-training (shifted 24 hr vs. 18 hr: t8 = 8.39, P<0.001; 24 hr vs. 30 hr: t7 = 7.67, P<0.001).
Within testing times, the phase shifted group consistently demonstrated reduced recall of contextual fear conditioned behavior than the un-shifted groups (18 hr: t8 = 5.09, P<0.001; 24 hr: t7 = 4.97, P<0.001; 30 hr: t7 = 6.03, P<0.001; Fig. 5B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com