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Correlation of spectral peaks with existing metabolite databases and chemical shift standards revealed primarily sugars and amino acids were present, in particular glucose and trehalose, as well as other metabolites such as succinate, acetate, and glycerol.
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Tetramethylsilane was used as an internal shift standard.
CDCl3 was used as the solvent and TMS as the internal chemical shift standard.
They were working a 24-hour shift – standard for Cal Fire crews – with no guarantee that they'd get a chance to sleep that night.
Tetramethylsilane was used as an internal chemical shift standard.
Sodium trimethylsilyl- [2,2,3,3-H4]-1-propionate (TSP) was added as an internal chemical shift standard (0.10% w/w).
To achieve on-the-fly recalibration of retention time shift, standard reference peptides (Pierce Peptide Retention Time Calibration Mixture, Thermo Fisher Scientific) with pregenerated iRT values (Table S1, Supporting Information) were spiked into samples.
All synthetic samples were prepared in D2O containing 300 μM NaN3 (to inhibit microbial growth) and 300 μM DSS (4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic
In a typical experiment, 20 mg of the TGM or MA-TGM were dissolved in 1 mL of D2O that contained 0.75 wt % TMP as an internal shift standard.
All dry metabolite extracts were dissolved in 800 μL (large samples) or 200 μL (small samples) of D2O containing 1 mM 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic 4,4-dimethyl-4-silapentane-1-sulfonic
A reigning cloud of shifting standards.
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