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Paramagnetic shift reagents allow for a clear separation between intracellular and extracellular sodium [15].
Chiral shift reagents, such as Eu(hfc 3, are still used to determine enantiomeric purity.
An application that has almost fallen out of use with the introduction of affordable superconducting magnets is the use of europium complexes, such as Eu(fod)3, as shift reagents in NMR spectroscopy.
Transport into or out of the vesicles can then be studied by techniques such as fluorescence (using ion-sensitive fluorophores), NMR spectroscopy (using shift reagents to distinguish between interior and exterior), or ion-selective electrodes.
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The one-dimensional (1D) H NMR spectrum of each compound was assigned, and the methyl was monitored throughout the titration with chiral shift reagent Eu- hfc 3.
Polysaccharides were stained with periodic acid shift reagent-PAS [ 79] counterstained with 0.02% Toluidine Blue for general structure, and proteins with 0.25% Naphtol Blue Black in 1% acetic acid [ 80].
The enantiomeric ratios, estimated from the H NMR spectra in the presence of a chiral shift reagent Chirabite-AR (Tokyo Chemical Industry Co., Japan), were 5 1 9 1 when the extent of enzymatic hydrolysis reached about a half.
The chiral lanthanide shift reagent Eu- hfc 3 (Sigma-Aldrich) was titratEu- hfc 3MR samples (all in CDCl3, Cambridge Isotopes Inc). of levosimendan, CMDP, AMDP, and CI-930 to determine wastitrated methyl on the pyrintoiNMRe ring wasamplesospecific or if the compounds were enallinmer mixtures (for a review, see ref (20)).
Reply: Please note in the original manuscript on p. 2 we stated that a shift to sustainable reagents (i.e. away from polyclonal antibodies) "would help to reduce the necessary financial and workload efforts associated with quality control of polyclonal antibodies and ensure sustainability", which partially addressed this point.
The authors concentrate on their core message for better characterization and documentation and for shifting toward renewable reagents.
Evidence for the residual BSA in the gels after in vitro release was provided by dyeing the gels with protein determination reagent and shift in the LCST of the gels.
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