Sentence examples for shift or phosphorylation from inspiring English sources
In HeLa cells transfected with a plasmid expressing dominant negative p38α, we failed to observe starvation-induced MK2 activation (i.e., an MK2 band shift or phosphorylation of its substrate HSP27), Beclin 1 S90 phosphorylation, or autophagy.
Moreover, at 1 µM IPA, which induces PERK activation, we found that Tm did not enhance the PERK mobility shift or eIF2α phosphorylation further than what was achieved by IPA alone.
In contrast, neither mobility shift nor phosphorylation of BIMEL was induced by ATO alone.
The phosphorylation shift (from baseline phosphorylation state), z, of a protein in reaction to stimuli x 1 … x n and inhibitor y is expressed by the stimulation-inhibition response function (SRF): z = F ( x 1, …, x n, y ), (5).
As expected, Mps1WT and Mps1as displayed a mobility shift and phosphorylation of T676 upon nocodazole addition in both HCT and UTR cell types (Fig. 1B).
It appears that the phosphorylated form of LASP-1 has more affinity over time though the human LASP-1 does not show a band shift upon phosphorylation [36].
The mobility shift and phosphorylation of S405/S418 are impaired by pharmacologic inhibition of mitogen activated protein/extracellular signal regulated kinase kinase (MEK 1/2, and biochemical evidence indicates that the MEK effector kinases ERK1/2 directly phosphorylate cortactin at these sites [40].
Protein band shift indicates phosphorylation.
DNA binding activity of the transcription factors NF-κB and STAT-3 was detected by eletrophoretic-mobility-shift assay (EMSA) and super shift, and phosphorylation of IκB-α and STAT-3 by Western blot.
The transition of RNAP II from the pre-initiation complex to the elongation complex is associated with a shift in phosphorylation of the C-terminal domain (CTD) serine 5/7 to serine 2 (Kim et al., 2010).
Hypoxic cells may utilise this pathway when low oxygen availability causes a shift from oxidative phosphorylation metabolism to non-oxidative phosphorylation metabolism.
