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For normal incidence, a sufficient phase shift difference between the two orthogonal polarization states of the reflected wave will be produced.
Instead of relying on chemical shift values of the methine peak, this new method was based on the chemical shift difference between CH(Bn) and CH B4) where the subscript "n" denotes the number of branch carbons.
Two FBGs of this sensor are bonded on the top and bottom surfaces of the cantilever, respectively, and the center wavelength shift difference between the two FBGs is used as a measuring signal, thus improving the pressure measuring sensitivity and avoiding any effect of temperature cross-sensitivity.
The HSQC spectrum was unhelpful in the assignment of these methylene signals, since the chemical shift difference between the C-6 carbons of interest is very small.
For rsp > 0.85, the phase shift is negative both on PB and matrix, and the phase shift difference between PB and matrix is small, which accounts for the obscure phase contrast obtained at rsp = 0.95 (shown in Figure 5a').
As rsp is in the region of 0.77 to 0.85, the phase shift becomes larger on the matrix than on PB, and the largest phase shift difference between PB and matrix was observed.
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However, different mobility shift, differences between polymeric forms of rTFF1/EC and rTFF1/BC on native PAGE gel and the unexpected mass increase of rTFF1/BC all indicated that other factors may contribute to the observed polymerization difference.
NMR studies have shown that the oxidized and reduced states of ERp18 have chemical shift differences between residues in and around the active-site disulfide, consistent with different conformational states [ 40].
(c) Averaged backbone amide 1H and 15N chemical shift differences between NTwt and NT* at pH 5.5 and pH 7.2.
The chemical shift differences between the two species are large, allowing unambiguous sequential NMR assignments to be made for both sets of resonances.
On the other hand, there are significant chemical shift differences between the spectra of [mo] q25 and [mo] r37 fibrils (Fig. 4c) as well as between the spectra of [Sha] q25 and [Sha] r37 fibrils (Fig. 4d), indicating that structurally distinct amyloid strains can be formed by these proteins depending on the experimental conditions.
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