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electrophoresis mobility shift assay.
Here we present a pressure shift assay (PressureFluor, analogous to the ThermoFluor thermal shift assay) that uses high pressure to denature proteins.
We have resorted to the thermal shift assay methodology to assess the predictions from VLS [13].
NF-κB activation and Oct-1 levels were determined by the gel shift assay.
Figure 1 Flowchart of the overall approach and the thermal shift assay results.
To this end, we performed peptide competition assays based on electrophoretic mobility shift assay.
The melting temperature (T m) for each mutant was measured using the thermo shift assay.
Binding capacity of PEI-GO toward siRNA was assessed by electrophoretic mobility shift assay (EMSA).
Activity of NF-κB in rat intestine was assessed by electrophoretic mobility shift assay (EMSA).
Hepatic NF-κB activity was examined by electrophoretic mobility shift assay (EMSA).
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These results validated our measurement of DNA binding activity of NF-κB by gel-shift assay.
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