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For bromo-deoxyuridine (BrdU) incorporation studies, QMb-ts transfected at 35°C were shifted to 41°C in DM and labeled with 20 µM BrdU for 2 hours at various times following temperature shift, as required.
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Skim occasionally as required.
Paint with as required.
Make adjustments as required.
Cut as required.
Do as required.
Make changes as required.
Adjust as required.
Paint inside as required.
"Everyone from the Scottish government to the HSE have approved of this and we will continue to shift the site as required and dependent on our needs".
I slowly established an imperfect sort of rhythm on each traverse, shifting my weight as required, a wobbling adjustment inserted here and there.
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