Sentence examples for shift analyses and from inspiring English sources

In addition to its well-established role in structural elucidation, we present recent examples that illustrate the combined power of selective isotope labeling, relaxation dispersion experiments, chemical shift analyses, and computational approaches for the characterization of conformational sub-states in proteins and enzymes.

We characterized the binding of a synthetic 73-amino acid peptide from human TTP to the tumor necrosis factor (TNF) ARE by gel mobility shift analyses and fluorescence anisotropy experiments.

While crystallographic analysis in a variety of space groups showed the Met20 loop to be in three dominant conformations, NMR data including chemical shift analyses and NOEs showed that in solution the enzyme is predominantly either in the "closed" conformation or in the "occluded" conformation and never stably in the "open" conformation.

Chemical shift analyses demonstrated that L53AI54A and YY contain native-like secondary structure in helices I and IV, while helix II is partly formed and helix III is absent.

Open image in new window Figure 1 CTCF-ZFs bind to M1 stronger than to M2. (A) The sequences of CTCF binding motif M1. (B) Gel mobility shift analyses between M1 motif and increased amount GST-CTCF-ZFs fusion protein.

Because mutation of the GATA element failed to reduce DNA binding in electromobility shift analyses (Fig. 4B), and because mutation of the Ebox reduced the AICAR effect to that of control values (Fig. 4C), we believe that PGC-1α expression is more likely to be regulated by USF-1 rather than GATA-4 in response to AICAR.

Subcellular fractionation and gel shift analyses revealed that SAAD increased the level of nuclear Nrf2 and activated ARE, which were also blocked by l-arginine deficiency or l-NAME.

To determine whether GATA3 protein was actively bound to the predicted consensus MUC1 promoter sequence (at -2398/-2393 from transcriptionalonal start site (TSS)) in vivo and in vitro, we performed ChIP and gel shift analyses in breast cancer cells.

Electromobility shift analyses (EMSAs) for GATA-4 and GATA-6 binding to murine uroplakins 1B and 2 promoter sequences were carried out as using methods previously described [39].

We have combined phylogenetic and function shift analyses to study the evolution of the RIFIN proteins, which are antigenically variant and are encoded by the largest multicopy gene family in Plasmodium falciparum.

We have combined phylogenetic and function shift analyses to study the Plasmodium falciparum RIFIN protein family.