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Extract (5 mL) at four different concentrations (0, 2.5, 5.0, and 10.0%) was placed into a sterile 9 cm Petri dish containing two sterile sheets of filter paper (Whatman No. 1).
The sections were dried for at least 2 days pressed between sheets of filter paper.
Finally, the membranes were washed in TBS and deionized water, and dried between two sheets of filter paper.
Additional segments were opened longitudinally and fixed flat between sheets of filter paper in 10% buffered formalin.
Leaves were weighted directly after sampling (wt0) and placed on sheets of filter paper in room temperature.
The opened intestinal segments were spread flat between sheets of filter paper and fixed overnight in 10% neutral buffered formalin.
Similar(49)
"2N" kinds of elements can be easily prepared by using "2N" sheets of filters (by considering the symmetrical nature of filters, "N" is sufficient) which have rather simple well patterns.
The method consists of applying the test solution or sample as a spot near one corner of a sheet of filter paper.
A sheet of filter paper, cut by hand, is fed into an $800 Xerox office printer that uses melted black wax instead of toner.
Partly for this reason, Martin and his coworkers worked out a new procedure in which the stationary medium was a sheet of filter paper.
Test samples (aqueous methanol extracts and a purified compound) were dissolved in a 0.2 mL of methanol and added to a sheet of filter paper (No. 2) in a 3-cm Petri dish.
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