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TNFS XPS table, cell response to varying TNFS GMA compositions, loss of cell morphology after detachment, comparisons of cell sheet transfer methods, transferred cell sheet viability, quantitative alignment computation schematic, and movies of cell sheet detachment and stained trilayer nanopatterned tissues and unpatterned controls.
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The detached cell sheet retained high viability and could proliferate again after transferred to a new culture surface.
As acridine is a volatile substance, a gastight cover sheet was used for viability studies (cf. [ 23, 33]).
These PLA/PANI conductive nanofibrous sheets exhibited good cell viability and promoting effect on differentiation of H9c2 cardiomyoblasts in terms of maturation index and fusion index.
Using light-sheet microscopy ensuring cell viability and stress-free root development during imaging, we found that RabA1d is involved in two essential cellular processes, cell plate expansion during cytokinesis and oscillatory tip growth in root hairs, representing highly active vesicle trafficking events.
Therefore, in the present study, we focused on both the maintenance of sheet structures and of cell viability after vitrification.
Thus, extra- and intracellular ice crystal formation is inevitable during freezing, which destroys the cell sheet structure and decreases cell viability [ 21].
Gel casted transferred cell sheets also maintained near 100% viability 24 h after transfer and cell growth continued outward from the edges of the cell sheets, albeit in a random growth pattern for the transferred nanopatterned cell sheet.
(D) Viability of cell sheets before and after preservation.
These results suggested that HBSS can maintain viability of cell sheets.
We estimated cell viability for flat sheets, and performed histological examination, analysis of extracellular matrix (ECM) deposition and mechanical tests on tubular constructs.
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