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Partly for this reason, Martin and his coworkers worked out a new procedure in which the stationary medium was a sheet of filter paper.
The method consists of applying the test solution or sample as a spot near one corner of a sheet of filter paper.
A sheet of filter paper, cut by hand, is fed into an $800 Xerox office printer that uses melted black wax instead of toner.
Test samples (aqueous methanol extracts and a purified compound) were dissolved in a 0.2 mL of methanol and added to a sheet of filter paper (No. 2) in a 3-cm Petri dish.
An aliquot of the extract (final assay concentration of tested samples corresponded to the extracts obtained from 0.3, 1, 3, 10, 30, 100 and 300 mg dry weight of B. brizantha shoots per mL) was evaporated to dryness, dissolved in a 0.2 mL of methanol and added to a sheet of filter paper (No. 2) in a 3-cm Petri dish.
The ash was dissolved in 20 % nitric acid, diluted in distilled water and filtered through a sheet of filter paper.
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Extract (5 mL) at four different concentrations (0, 2.5, 5.0, and 10.0%) was placed into a sterile 9 cm Petri dish containing two sterile sheets of filter paper (Whatman No. 1).
The sections were dried for at least 2 days pressed between sheets of filter paper.
Finally, the membranes were washed in TBS and deionized water, and dried between two sheets of filter paper.
Additional segments were opened longitudinally and fixed flat between sheets of filter paper in 10% buffered formalin.
The opened intestinal segments were spread flat between sheets of filter paper and fixed overnight in 10% neutral buffered formalin.
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