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Briefly, melted gelatin is added to the TNFS which is incubated at room temperature and then cooled to solidify and encase the detaching cell sheet prior to full sheet detachment.
Additionally, during cell sheet detachment from PNIPAM, deposited ECM proteins are also detached intact from the surface.
The ability of cell sheet detachment was related to the extent of thermosensitivity.
Faint ECM remnants were observed on the dish surfaces after cell sheet detachment.
NIH 3T3 mouse fibroblasts served as a model cell line for adhesion, proliferation, and cell sheet detachment.
The rough surface texture of the semi-IPN NC gel containing AL accelerated the cell sheet detachment, due to faster water penetration.
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Lowering the temperature to 4 °C and 20 °C below TT of each brush led to the cell-sheet detachment as a monolayer form from the polymer brushes accompanying with the switching of surface affinity.
A cold LED UV light which could eliminate heat interference with 365 nm wavelength was used in this study for cell sheets detachment.
Ice adhesion experiment on the superhydrophobic sheet showed ice detachment strength at approximately 20 kPa on average, which was < 2% of the ice detachment strength of an aluminum substrate, 33% of the ice detachment strength of a smooth non-textured Teflon FEP sheet and among the lowest superhydrophobic ice adhesion strength reported in literature.
We examined a wide range of processing parameters in order to identify the conditions that led to acceptable sheet growth and detachment behavior.
These parameters can thus be exploited to either inhibit or purposefully induce a collective detachment of sheet-like microtissues for the use in tissue engineering and regenerative therapies.
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