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The ultra-low reflection material of CuO/Cu in UV vis is designed and achieved via orderly growing ultralong CuO nanowires on copper sheet, constructing the ultra-thick and vertically aligned array.
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a Schematic diagram of a perfect graphitic carbon nitride sheet constructed from melem units.
For obtaining a stable stacked cell sheet construct, a sophisticated 3-D cell sheet manipulation system using temperature-responsive culture dishes and a cell sheet manipulator was developed.
Moreover, the endothelial networks in the five-layer myoblast sheet construct were observed to connect to the host vessels after transplantation into the subcutaneous tissues of nude rats, resulted in a neovascularization that allow the graft to survive.
To observe the 3D network structure inside the cell sheet construct, an 8 μm thick cross section was cryosectioned.
Our main aim in this study is to investigate the vascularization ability of a 3D HUVEC/hMSC cell sheet construct.
However, the in vivo regeneration abilities of this cell sheet construct for specific tissues still need to be further investigated.
The cell sheet construct was fixed in 4% paraformaldehyde solution and prepared the same embedding procedures as described in Section 2.2.
Results suggested that the 3D prevascularized HUVEC/hMSC cell sheet construct provides a promising potential as a vascularization platform for wide applications in tissue regeneration.
We hypothesized that this prevascularized 3D cell sheet construct can promote the formation of blood vessels and functional anastomosis with host vasculature.
Therefore, the aims of this study are to develop a highly prevascularized 3D cell sheet construct through seeding HUVECs on an hMSCs cell sheet and to investigate its vascularization potential in vivo.
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