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WA1 and 95/1000 contained three FlaA sheath protein genes while 50-150T contwoned TableTable 3).
The subsurface proteins, endoflagellar sheath protein, FlaA1, and the subsurface protein, LipL31, were used as negative controls for surface proteolysis (Fig. 2E).
The three dimensional fold of VCA0108 and VCA0107 showed some similarity to phage tail sheath protein and a protein ImpB implicated in protein secretion respectively.
Control analysis of the endoflagellar sheath protein FlaA1 (which is attached to the inner membrane) confirmed that the detergent phase was not contaminated with inner membrane components (Fig. 2B).
The tpr genes are found in several strains of Treponema pallidum and also in T. paraluiscuniculi, and their paralogous proteins are sequentially related to the major outer sheath protein (Msp) of Treponema denticola [35].
Within the 2 myophages left without any assigned Sheath protein, C-st was experimentally shown to be a myophage and should have a Sheath protein.
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Finally, regarding Sheath proteins, the 3D structures are known for phages T4 [ 46] and PhiKZ [ 47].
We first searched for the major capsid proteins, portal proteins, terminases, major tail proteins and Sheath proteins within Aclame 0.4.
70 Sheath proteins were identified starting from the T4 protein, out of the 74 Myophages in Aclame.
Only 2 Sheath proteins were identified starting from the PhiKZ protein, corresponding to the giant phages PhiKZ and PhiEL.
In order to construct a phylogenetic tree using single gene products, putative major capsid proteins and tail sheath proteins were identified from all 61 phages.
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