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An undulating notochord between 12 and 24 hpf can be a consequence of defects in either sheath assembly or vacuolation.
Moreover a crossover introducer sheath assembly creates more possibilities for easier catheter-guidewire manipulations.
TssB and TssH no longer formed foci in the ΔtssE mutant, consistent with a lack of sheath assembly and contraction.
Indeed, TssM, the other inner membrane protein with which TssL tightly associates (Ma et al., 2009), is required for TssB sheath assembly (Kapitein et al., 2013).
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Further, we show that four core T6SS components, TssB, TssH, TssJ, and TssL, all exhibit distinct behavior in vivo and provide support for a model of T6SS assembly, whereby the contractile sheath assembles at a subset of potential sites defined by the membrane complex in anticipation of firing.
Similarly, the sleepy and grumpy loci encode laminin chains which, when defective, cause aberrant assembly of the trilaminar sheath [47].
The participation of the secretory pathway in notochord formation is well established in zebrafish; extracellular matrix biosynthesis and constitutive exocytosis are essential for correct assembly of the perinotochordal sheath [43], [46], [47].
Although lipid metabolism was not studied in the brain in this study, we postulate that the disturbances in lipid metabolism might affect the assembly of the myelin sheath, leading to defective myelination.
This work also provides important evidence as to why translocation of the mRNA that encodes myelin basic protein is important in the assembly of the myelin sheath since premature synthesis seems to provoke aberrant, ectopic compaction of the myelin membrane.
It is thought that TssE, with other components of the T6SS basal complex, might contribute to the assembly of the Hcp tube and the TssB TssC sheath structure [ 23].
AKAP 3 is the basic organized structure of the sheath while AKAP 4 plays an imperative role in completing the assembly of fibrous sheath [ 119].
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