Exact(4)
These two loci are not bound by Sir proteins and hence were not expected to change in shearing size in Sir− cells.
Of the 621 peakspeaks, 76 (12%) were ≤300 bp, the shearing size of the ChIP DNA, from a G4 motif.
For Pfh1 and Cdc20 peaks, a window of 300 bp, the DNA shearing size, was used, but our main results held with windows of 0 and 500 bp.
Peaks were identified with MACS1.4 [ 49] using the following settings: bandwidth of 300 (DNA shearing size), P-value cutoff of 10-5 and tag size of 101.
Similar(56)
After ultrasonic DNA shearing, size-selection is carried out via double solid phase reversible immobilization (dSPRI) using carboxyl coated magnetic beads (Figure 1).
For FLX sequencing, the blunt fragments produced by AluI digestion were ligated directly to the adapters provided in the library preparation kit without the shearing, sizing or end-polishing steps.
Due to its shear size, complexity, and plastic nature, dissecting the high-resolution three-dimensional structure of the NPC in its hydrated state is a formidable challenge.
Wind velocity gradients or horizontal wind shear (size of cyclone, the intensity of cyclone, proximity to the cyclone, and local scale cyclonic convection effects).
Studies of the MUC1 extracellular domain (ECD) are complicated by its shear size (150 300 kDa), the fact that it is highly glycosylated, and that it is made up of a variable number of tandem repeats.
For multiplex sequencing, DNA samples, each with a different barcode, are pooled, randomly sheared, size selected (300 700 bp), and a second adapter is ligated after polishing and filling ends.
These distances are significantly shorter than the average shear size of the target DNA, suggesting that the shear size does not strictly limit the resolution on the tiling array due to presence of signal peaks in the data.
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