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Due to its shear size, complexity, and plastic nature, dissecting the high-resolution three-dimensional structure of the NPC in its hydrated state is a formidable challenge.
Due to the shear size of many challenges in computational science, the use of supercomputing, parallel processing, and - phisticated algorithms is inevitable and becomes a part of fundamental theore- cal research as well as endeavors in emerging ?elds.
Wind velocity gradients or horizontal wind shear (size of cyclone, the intensity of cyclone, proximity to the cyclone, and local scale cyclonic convection effects).
Studies of the MUC1 extracellular domain (ECD) are complicated by its shear size (150 300 kDa), the fact that it is highly glycosylated, and that it is made up of a variable number of tandem repeats.
These distances are significantly shorter than the average shear size of the target DNA, suggesting that the shear size does not strictly limit the resolution on the tiling array due to presence of signal peaks in the data.
This may result either from weak binding of TBP to these regions or from variability in shear size of ChIP DNA.
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These two loci are not bound by Sir proteins and hence were not expected to change in shearing size in Sir− cells.
For multiplex sequencing, DNA samples, each with a different barcode, are pooled, randomly sheared, size selected (300 700 bp), and a second adapter is ligated after polishing and filling ends.
Of the 621 peakspeaks, 76 (12%) were ≤300 bp, the shearing size of the ChIP DNA, from a G4 motif.
Peaks were identified with MACS1.4 [ 49] using the following settings: bandwidth of 300 (DNA shearing size), P-value cutoff of 10-5 and tag size of 101.
The N50 size is two times higher when using the 20 kb library, suggesting that we obtained longer MinION® reads when sheared size is increased.
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