There was sharp cell membrane staining in the absence of saponin, but only approximately 10% of the cells had this membrane staining, which we cannot explain, but presumably is due to a problem of accessibility and/or fixation.
However, a somewhat injured epithelial structure was observed in the healed corneal tissues of group 1. TEM revealed a sharp basement membrane structure composed of lamina lucida and lamina densa with some hemidesmosomes in all three groups.
Those areas with no sharp outlined membranes were discarded.
Conceivably, upregulation of both JC15 and CypD resulted in a sharp decrease in membrane potential, which overlapped with the mitochondrial calcium release.
Between concentrations of 1.25 μg/ml and 5 μg/ml, no difference in morphology and spontaneous beating could be observed, whereas at concentrations higher than 5 μg/ml cells appeared round and increased in size, no sharp intact cell membrane could be detected and most cells had died.
Sharp ribbon-like membranes delimited DIMs (Fig. 3c) that mostly consisted of open structures (Fig. 3c), suggesting intrinsic membrane rigidity.
In addition, the designed glass substrates of Au/GCNE were mechanically stronger and their sharp tips aided in membrane penetration during implantation in the in vivo experiment.
There is a high possibility of puncturing the membrane by sharp angular glass particles during placement and compacting.
PVdF membrane has sharp peaks at 20.7° (110) and 26.60° (022) corresponding to orthorhombic α-phase of PVdF [30].
Unexpectedly, we notice a very sharp depletion of basement membrane VEGF165-HSPG (from 540 pM to 160 pM) (not shown).
