Determination of borders of structures in fluorescent images is, however, somewhat subjective because borders do not appear sharp at higher magnifications (e.g., ×10 or ×20 magnification).
For H needles, only one needle (H8) exhibited a relatively smooth and sharp end, and the best magnification for the SEM observations was 5000×.
The obtained bone samples were rinsed extensively with phosphate-buffered saline (PBS PAA Laboratoryy, Pasching, Austria) and cleaned with sharp instruments under the 10-fold magnification of a light microscope.
This offers several advantages since contrast, brightness, and magnification can be adjusted digitally, and a sharper and more detailed view is therefore obtained.
Following polymerization, the region of interest was checked employing low-magnification lens; using the point of a sharp scalpel the areas of interest (GPe and GPi) were cut out.
In this system, each of the neurons sampled by 400× magnification was visualised digitally, focused, and the sharpest and longest profiles of the nucleus and AgNOR area were traced by the mouse cursor on the screen.
Low magnification images show, in common with optical microscope techniques, sharp circular growth fronts which move at a constant growth rate.
i Histopathological examination revealed broad, thin-walled, non-septate hyphae, which are characteristic of Mucormycete (left arrowhead) and the other septate fungal hyphae branching at a sharp angle, which are characteristic of Aspergillus spp. (right arrowhead) (magnification: 400×).
Higher magnification SEM imaging of silica microglobules revealed a rough surface entirely comprised of sharp-edged triangular and hexagonal platelets of 1 3 µm edge length and 100 300 nm thickness (Figure 4b).
20 Although it is tempting to attribute this to differing IOL material, methods using high magnification (500 1000×) scanning electron microscope photographs have recently shown that hydrophilic acrylic IOLs marketed as having sharp edges show significantly rounder optic edges than sharp-edged hydrophobic acrylic and silicone IOLs.
