To subclone, cDNA from the first instar larvae was used as a template to obtain a 527-bp PCR fragment of the shared common domain, and two full-length cDNAs were obtained with a combination of 3'-RACE and 5'-RACE methodologies.
In particular, we introduced the concept of "domain clubs", which are sets of proteins that share common domain compositions.
Paraspeckles are one of the most recently identified nuclear bodies and contain a family of RNA-binding proteins called the DBHS (Drosophila behavior and human splicing) family proteins, which share common domain structures consisting of three RNA recognition motifs arranged in tandem (Fox et al., 2002; Bond and Fox, 2009).
ERp57 is essential for efficient folding of glycoproteins sharing common structural domains, for instance Type 1 transmembrane proteins, such as α-sarcoglycan, and proteins that possess EGF-like domains, such as β-, γ- and δ-sarcoglycan (Ref. 127).
Structures of TCR pMHC complexes have not shown conserved contacts that could mediate an MHC bias, although structures of different receptors sharing common variable domains suggest types of interaction that may be conserved between particular gene segment MHC pairs [ 8, 71], and, as noted above, recent results are compatible with the germline β-chain loops driving the initial pMHC contact [ 7].
Minicollagens share common domain architectures with a central collagen sequence flanked by polyproline stretches and short N- and C-terminal CRDs.
11 These members all share common domain structures, including N-terminal HEAT Huntingtin, elongation factor 3, protein phosphatase 2A, and yeast kinase TOR 2 (HEAT) repeats, a FRAP, ATM, TRRAP (FAT) domain, a protein kinase domain, and a C-terminal FAT-C domain.
It is hypothesized that cancer proteins, also known as tumor associated genes, may share common functional domains [ 13], and thus a weighted domain score for each tumor associated gene's domain is determined.
Even if only the distinct domains are used, the subsets are still not conditionally independent because they share common pairwise domains.
The YjeK homologs share common enzymatic domains with a protein involved in lysine catabolism, lysine aminomutase (LAM).
