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We note that the BGVM procedures can be applied to identify shared TFBSs, shared coding or non-coding genes and shared repeated elements between circular genomes.
Separate files containing the concatenated sequences of the intergenic regions of Leptosira and Chlorella cpDNAs were produced to search for the presence of shared repeated elements = 30 bp with up to 10% mismatches using the -d -p -l 30 -e 3 -seedlength 10 -q -v options of V match [ 46].
However, we note that our procedures can be applied to many features of genomic organization, e.g. shared TFBSs, shared repeated elements (Benson and Waterman, 1994) and shared non-coding genes (e.g. rRNA and small RNA), by inputting their corresponding angles instead of those of shared orthologs to our algorithm.
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After removing the shared repeats, the remaining sequences were annotated using the all-plant repeat database.
It coincides well with the fact that the shared repeat sequences, but with different amount of such fragments, among the Glycine species.
The location indicates position of shared repeats.
The largest shared repeat (49 bp) represents shared sequence between psaA and psaB.
Here, we defined repeats that had identical lengths and located in homologous regions as shared repeats.
The majority of the shared repeats were located within intergenic spacer regions and introns.
IR: inverted repeat; T: tandem repeat; – no repeat; 0 gene absent from cpDNA ; * shared repeat; # located 5' to the gene.
Repbase update release 13.05 contains only 145 ancestral shared repeats and one lineage-specific repeat for rainbow trout, and for salmon, 141 ancestral shared repeats and five lineage-specific repeats [ 36].
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