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These fragments shared complementarities with the ends of a linker DNA, the central part of which is the target for active DNAzyme.
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To confirm its directionality, cDNA was synthesized for subsequent PCR amplification using a primer located within the 3'UTR exon of grna that shared complementarity to ASgrna (sense relative to grna) or using another primer that was located downstream of the cloned ASgrna sequence and within a known intron for grna (Additional File 11, panel A).
A large proportion of lncRNAs are transcribed in antisense orientation of protein-coding genes, with which they often share sequence complementarities [ 8, 9].
We have identified several mRNAs that share complementarity with the unique sequence of PrrH by in silico analysis using Target RNA (http://snowwhite.wellesley.edu/targetRNA/).edu/targetRNA/
The fact that these NATs share complementarity with their cognate mRNAs make the related piRNAs and endo siRNAs perfect guides to target effector complexes to protein-coding transcripts.
These noncoding RNA elements are around 21 bp long and bind the target mRNA sequences which share complementarity with the targeting miRNA sequences.
The following paragraph explains the proposed control mechanism in a scenario where a protein-coding mRNA shares complementarity with a processed antisense transcript; nonetheless, the mechanism could be relevant to other transcriptional units that produce complementary RNA.
The motivation for the actors to commit to innovation does not only lie in the functional complementarities (shared functions) but also in the unique functions of each sphere.
The cis-encoded sRNAs are encoded at the same chromosomal locus as their regulated mRNA but on the opposite strand and thus necessarily share extensive complementarity with the corresponding transcript.
Three unidirectionally cloned ESTs (accessions CD585878, CD585963 and CD596001) were all revealed upon sequencing to correspond to a 914 nucleotide long cDNA sharing perfect complementarity to nucleotides 2701 3614 of the grna cDNA (Additional File 11, panel A).
MicroRNAs (miRNAs) regulate gene expression by recruiting the RNA-induced silencing complex (RISC) to a target mRNA with which it shares partial complementarity, causing a reduction in the stability of the mRNA and/or its rate of translation [ 6].
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