Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank.
In fact, these proteins share a very high sequence similarity with the mammalian K+-dependent Na+/Ca2+ antiporter NCKX6 (Cai and Lytton 2004; Shigaki et al. 2006).
To further determine the cellular Ubc13 protein level under various stresses, we used the monoclonal antibody 4E11 produced in-house against hUbc13 (Andersen et al. [2005]), which shares a very high degree of amino acid sequence identity with OsUbc13 (Figure 1a).
Within the cotton clade, three paralogues, GaSus1, GaSus3 and GaSus4, which share a very high degree of sequence similarities, grouped more closely in the phylogenetic tree.
Such a signal was identified in CdtB of A. actinomycetemcomitans, which shares a very high protein sequence homology (97% identical amino acids) with CdtB from H. ducreyi used in this study.
The zebrafish MMP14b and MMP14c genes share a very high sequence identity and appear to have evolved from a recent intra-chromosomal duplication of seventeen genes on chromosome two (Fig. S6 in Additional file 4).
The three human PrsA isoforms share a very high sequence identity (91 95% between all three); however, the subtle differences that do occur appear in the regions of the enzyme that are important for catalysis and allosteric regulation.
Comparative analyses of the full genomes showed that the strains share a very high (99.5%) identity with the new variant OH851 (GenBank accession no. KJ399978) recently reported from the United States (6 ).
Among 11 zinc-dependent HDAC isoforms, it is particularly challenging to achieve isoform inhibition selectivity between HDAC1 and HDAC2, since they share a very high sequence similarity (97.8%, see Figure S2), have the same conserved residues around the catalytic pocket (see Figure 1), and the RMSD of two aligned protein crystal structures is only 0.7 Å (see Figure S2).
At last report, NovaStar had 9.5 million shares outstanding, and a short position of 8.1 million shares, a very high proportion.
