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In the shape of the branches we can recognize an older or younger tree.
Polyp density was estimated by counting the number of polyps/cm2 and standardized with branch thickness, assuming cylindrical shape of the branches.
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Therefore, it is reasonable to imagine that the shape of these branches can influence how the neuron works; however, this idea has rarely been tested experimentally.
We used landmark-based geometric morphometrics to measure the shape of the ventral branches.
We used these five landmarks to quantify precisely and accurately the shape of the ventral branches.
The most conspicuous difference in genital morphology that has been reported between D. yakuba and D. santomea is the shape of the ventral branches that cover the aedeagus (Kamimura and Mitsumoto 2012a; Kamimura 2012; Yassin and Orgogozo 2013).
These landmarks, although not documenting the shape of the ventral branches at their base, where they emerge from the aedeagus, allowed us to describe and quantify the shape of the distal end of the ventral branches, and especially the relative length and width of the spines to identify the main axes of shape variation in our QTL mapping population.
Here, we perform the first QTL mapping of genitalia divergence between Drosophila yakuba and D. santomea, and we focus on a trait that has been linked to reproductive success, the shape of the ventral branches.
To identify the genetic loci underlying this genital difference, we developed a method to measure the shape of the ventral branches in a quantitative manner and used it for QTL mapping in a D. santomea backcross population.
Briefly, the principle of QTL mapping is to correlate segregating genetic markers with trait values to identify chromosome regions that significantly affect the phenotype of interest, here the shape of the ventral branches, when substituting a D. santomea allele for a D. yakuba allele.
The geometrical shape of the individual branch is a cone.
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