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Using Cell Profiler software we quantified eccentricity, form factor and compactness, all of which measure shape, of nuclei expressing TDP-43 in comparison to control nuclei, which appear round and smooth.
The changes in the size and shape of nuclei of the Hoechst 33342 stained cells can be observed under fluorescence microscopy.
The resulting dispersions in size and shape of nuclei are obtained by multi-dimensional maximization of Gibbs free energy in morphology domain of potassium acid phthalate.
As a whole, the number of vacuoles increased, shape of nuclei was irregular, and cytoplasm and nucleus were less dense.
Technique 2 is advantageous with respect to platelet lineage assessment: megakaryocyte counts were generally significantly higher, and details in the shape of nuclei were easier to see.
4, 5 Features recognizable from EC include the shape of nuclei and the morphology of gland duct lumens in the superficial epithelial layer.
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In addition, the shape of nucleus was irregular, with evidence of mitochondrial overflow after cell death.
The micrograph of Ix however shows a slight aberrant shape of nucleus with shrinking in size and thickening of membranes with irregular nuclear membrane.
The ultrastructure of the HL-60 cell is similar to that of the early promyelocyte in size and shape of nucleus, structure of chromatin and the presence of well-developed Golgi apparatus [ 20].
Interestingly, the shapes of nuclei of secretory cells were always found to be irregular in both species.
Typically, within minutes of wounding we observed detectable effects on the shapes of nuclei, fusiform bodies and the aggregation of GFP-Nit1.
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