The shape of sal mutant clones in the central region of the wing blade is rounded, suggesting that sal mutant cells tend to minimize their contacts with wild type cells (Fig. 3B, Fig. 5A) (see also Milán et al., 2002).
The technique consists of two parts: a kinase containing a space-creating mutation in the ATP-binding pocket and a bulky ATP-competitive small molecule inhibitor that complements the shape of the mutant ATP pocket.
If you fit this bill, you better be keeping a close eye on performance art darling Kalup Linzy, whose portfolio takes the shape of a mutant hybrid of high brow art and low brow TV in the best of ways.
E-cadherin staining showed irregular elliptical shape of the mutant collecting ducts (Fig. 3C).
The altered intersection mode and shape of the mutant epithelial cells were also accompanied by aberrant cell alignment.
The expressivity of the phenotypes also depends on the size (fewer than 5 cells versus more than 5 cells) and on the shape of the mutant clones (round versus drawn-out) (compare Fig. 3A with Fig. 3B).
This is reflected in the shape of sal mutant clones, which are rounder than normal (Milán et al., 2002), and in the formation of epithelial folds at the place of confrontation between sal-expressing and sal-non-expressing cells located in the central region of the wing.
Because the shapes of many mutant cells are not regular, we therefore named the cells by their interfaces (int).
In medium containing 0.05 mM IPTG and 3 mM MgCl2 the shape of the Leu294Asp mutant growth curve differed from that of the wild-type TmCorA: the mutant cells grew significantly slower, reaching only an OD600 of 0.6 (~ 50% of the wild-type CorA) (Fig. 4c), which indicates a Mg2+ overdose caused by the constant Mg2+ uptake depicted in Fig. 3.
The single enlarged unbudded cell shape of the rad2C65Δ mutant is similar to the morphology of cells deficient in bud formation due to a defect in Cdc42p, which is essential for yeast actin cytoskeleton polarization (Pruyne and Bretscher, 2000a).
The leaf shape of doq-1 mutants was also similar to that seen in an-1, an-2, an-EM1 mutants.
