Likewise, the scrambled sequence (5′-TCT TAA TCG CGT ATA AGG C-3′) was used to construct the sham vectors that served as negative controls.
As shown in Figure 3c, melatonin treatment induced a significantly enhanced inhibition of cell invasion (73%) in cells that are transiently transfected with and that overexpress MT1 as compared with sham-transfected and vector-transfected cells (approximately 40% inhibition).
BB151120 was sham "complemented" with either an empty vector (pLAFR2) carrying the tetracycline marker or truly complemented with pLAFR2 containing a wild type luxCDABE allele (under control of its endogenous promoter; Figure 3B).
Three hours after injection (Fig. 1), no sign of cuticle sclerotization was observed in abdomens injected with 0.5 μl of the supernatant from a cell culture transfected with blank vector (Fig. 1a, sham control) or with r-bursicon α (Fig. 1b, negative control) or with r-bursicon β alone (Fig. 1c, negative control).
Methods: Peanut-allergic C3H/HeJ mice received 0.9 (low dose), 9 (medium dose), or 90 (high dose) μg HKE-MP123 pr, HKE-containing vector (HKE-V) alone, or vehicle alone (sham) weekly for 3 weeks.
Compared with Sham, the expression of Myc tag was detected at empty vector group.
Sham transfected PC-3M cells were created using an empty pcDNA3.1 vector as a control.
After 1 week, the tumor developed and 1 × 10 NSC vectors were injected into the tumor site and into sham injection site, respectively.
In addition, there was another interesting result that apoptosis rate in Sham group at 6 h p. i. was higher than that in empty vector group.
For silencing of IKKα, mice were divided into four groups (n=6 each): (1) ischemia-reperfusion and recovery (IR); (2) IR treated with a GFP-IKKα-encoding lentivirus (silence vector); (3) IR treated with GFP-encoding lentivirus (control vector); and (4) sham-treated group, in which the left renal pedicle was bluntly dissected without clamping.
