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After hybridization, the slides were immediately placed in wash solution 1 (1×SSC and 0.1% SDS) to remove the cover slip and washed by gentle shaking in solution 1, 2 times for 5 min each; then washed in solution 2 (0.1×SSC and 0.1% SDS), 2 times for 10 min each; and finally in solution 3 (0.1×SSC), 4 times for 1 min each.
The magnetic particles can be brought back into the original solution by removing the external magnetic field and gently shaking the solution.
Serial dilution was done by shaking the solution for 15 min, and then filtered using a sterile cheese cloth.
After shaking, the solution was allowed to settle for 30 min, filtered, and analyzed for cadmium by atomic absorption spectroscopy.
Foaming property was measured by the height of foam after shaking the solution of gemini in hard water (hardness 160 mg/L).
The formed structures are unstable and are destroyed by the light shaking of solution, after which the formation and growth of fractal structures is restarted.
After shaking, each solution was filtered using a ceramic sieve, and the absorbance of the filtrate was determined at the appropriate λmax value for the determination of the remaining dye content to optimize the shaking time.
The same experimental conditions were carried out to study the effect of time by shaking the solution for 10 h and the filtrate concentration was measured every 2 h by atomic adsorption spectroscopy.
The OPP sample was then extracted by adding 1 ml of ethyl acetate, shaking the solution well and then allowing it to settle.
Na-deoxycholate was added to a final concentration of 1% (w/vol) and after vigorous shaking the solution was incubated at 8°C for 6 h.
What they decided in the end was a head-shaking solution.
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