Sentence examples for shaking heat from inspiring English sources

Tissue samples were incubated overnight at 56°C with proteinase K and lysis buffer in a shaking heat block.

Cells at a concentration of 5 × 10/ml per condition were incubated at 37 oC on a shaking heat block (for times and reagent concentrations see figure legends).

Cells at a concentration of 2 × 10 cells/ml per condition were incubated with H2O2 (Thermo Fisher Scientific), Q-VD-OPh (R&D Systems) or combinations of these at 37 °C on a shaking heat block for 4 h.

Samples were heated at 95°C for 2 min. The mixture was transferred to a 48°C shaking heat block (Eppendorf Thermomixer; Eppendorf North America, Inc., New York, NY) for 1 hr.

Neutrophils at 2.5 × 10/ ml were incubated with treatments in 2 ml Eppendorf tubes on a 37 oC shaking heat block for the indicated time-points and pelleted by centrifugation at 300 × g for 4 min before resuspension and transfer to coverslips.

Neutrophils were resuspended in HBSS with cations and incubated with or without the flavones (all at 50 μM) on a shaking heat block for 30 min before addition of N-formyl-Met-Leu-Phe (fMLP; 10 nM; Sigma) or control medium for a further 15 min. Neutrophils were then incubated with appropriate antibodies (CD11b, Biolegend, Cambridge, UK; CD62L, BD Pharmingen) and analyzed by flow cytometry.

Loading of the drug and MNPs into the micelles was done by shaking, heating, and then sonicating the mixture [ 12].

Both supernatants were pooled, and 8 μl of 5 M NaCl was added and incubated at 65°C over night at 950 rpm on a shaking heating block.

Proteinase K was added to a final concentration of 1 mg/ml and incubated at 56°C for 2 h at 950 rpm on a shaking heating block.

DNA was eluted twice by adding 100 μl ChIP elution buffer (50 mM Tris, pH 8.0; 1 mM EDTA; 0.1% SDS; 50 mM NaHCO3), incubating at 65°C and 1,200 rpm on a shaking heating block for 15 min followed by centrifugation at 13,000 rpm for 1 min at room temperature.

"Absolute madness," Riley called Game 6, minutes after Madison Square Garden stopped shaking, the Heat having squandered a 15-point lead in the second half.