Sentence examples for shaking diluted from inspiring English sources

Before use, bacteria were incubated overnight at 37°C with shaking, diluted 100× in fresh media and incubated in the same conditions for 3.5 hours.

For E. coli, strains were grown overnight at 37°C in liquid LB medium with shaking, diluted into LB with the indicated concentrations of metalloid, and allowed to grow with shaking for an additional 8 hr.

The dried extracts were resuspended with 60  μL of methanol, vortexed, shaken, diluted with 40  μL of 60 mM aqueous ammonium acetate solution, transferred into the autosampler, and subsequently analyzed.

Overnight cultures grown under selection at 37°C with shaking were diluted 1000 × in 5 ml LB with or without selection, and aliquots of the time zero cultures were diluted and plated onto LB plates containing X-Gal (20 μg/ml, Saveen).

Spinner cultures were grown at 27°C at 140 rpm shaking and diluted to 2×106 cells/ml for infection.

KO and Inv2 cells were grown in 15 ml 2XPP for two days, spun down, resuspended in 5 ml starvation media (10 mM Tris, pH 7.5), incubated overnight without shaking, and diluted to 2.0×104 cells/ ml.

UTI89 was grown overnight in LB broth at 37°C with shaking and diluted 1∶1000 in LB or LB with varying amounts of methyl α-D-mannose or heptyl α-D-mannose.

Presence of saponins was detected based on the formation of froth upon vigorous shaking using diluted samples.

Unless otherwise indicated, all the strains were grown routinely in YPD medium (i.e., 1% yeast extract, 2% peptone, and 2% dextrose) at 30° with shaking overnight, diluted to an OD600 of 0.1−0.2, grown to logarithmic phase, and used for subsequent experiments.

Presence of saponins was detected based on the appearance of froth upon vigorous shaking of diluted samples.

Diluent (60 mL) was added and the flask solution was sonicated for 10 min at 10 °C with intermittent shaking and diluted to volume with the diluent.