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The teacher told me she still hadn't cried, although sometimes she shakes with cold.
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Now she is shaking with cold as well.
One moment I felt I was in a furnace, the next I shook with cold.
Oates responded by "shaking with cold, with a kind of choked fury".
"You have problems up here for which one was not prepared by getting a Ph.D. in art history," Nan Rosenthal, who oversees the installation of sculptures in the roof garden, said as she stood outside smoking and shaking with cold.
The youngest boy, Ivan Ivan Dobronravovv), is too chicken, or too wise, to jump, and he stays up there, shaking with cold and shame, until his mother climbs up and folds him in her arms.
Then, shaking with cold, Donny folded his arms around himself and yelled out, Hey, and we heard back, Hey, hey, hey, and then I yelled out, Hey, and even Mr. Cheetam joined in, and we kept hearing back, Hey, hey, hey, like there were millions of us everywhere.
He was shaking with the cold and the effects of the chemo, but he wanted to carry on.
For the preparation of developmental RNA, the developing cells were detached from the membranes by incubation in cold PBS containing 5 mM EDTA for 5 min followed by vigorous shaking, and washed twice with cold PBS.
The DP were 1) MET: digesta was incubated at 38°C for 15 min with saline solution (0.9% NaCl) containing 0.1% methylcellulose under continuous shaking; 2) STO: digesta was mixed with cold saline solution and homogenized with a stomacher for 5 min at 230 rpm; 3) FRE: digesta was immediately frozen at −20°C for 72 h, thawed at 4°C, mixed with saline solution and subjected to STO procedure.
Drain, refresh with cold water and drain again, shaking the sieve to get rid of all the excess water.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com