The shaking device was a desk-type constant-temperature oscillator (THI-92A, China).
Working on the principle that the residence time of a particle on a sieve is a function of the particle shape, the cascadograph consists of a stack of sieves of identical size in a shaking device.
Then we've made an anti-shake device for a tracking shot of the desert.
The PAM was calibrated on a shaking device (2.1 kHz) prior to the study.
Peptide synthesis was carried out using a multiple peptide synthesizer (Intavis) equipped with a shaking device under standard conditions.
Peptides were synthesized on an Intavis MultiPep RS peptide synthesizer with a shaking device following slightly modified standard synthesis protocols.
Bruising tests were performed in duplicate between December (very early and early genotypes) and January (mid early to late genotypes) by exposing 8-12 kg tubers to mechanical impact in a rotating washing machine or other shaking device.
As in previous experiments [ 10, 11], specimens were immersed in 500 μL dH2O, in sealed polystyrene 48-well plates (Costar, High Wycombe, UK) at room temperature on a shaking device (180 rpm).
Sepharose beads (40 μl per well; Streptavidin Sepharose High Performance, Amersham Biosciences, Uppsala, Sweden) were diluted with binding buffer, mixed with the PCR product, transferred to a filter plate, and incubated for 10 min at room temperature on a shaking device with continuous mixing.
Precultures were cultivated in 125 mL baffled Erlenmeyer flasks (Corning Inc., Germany) at an initial cell density of 0.4×10 cells/mL and a working volume of 50 mL on a shaking device (225 rpm) at 37°C and 5% CO2 in a humid atmosphere.
To generate working cell banks (WCBs), 10 μL of each MCB was added to 990 μL medium with 40 g L 1 D-glucose in a Flowerplate® (mp-labs, Baesweiler, Germany) and incubated for 48 h in a shaking device at 1000 revolutions per minute (rpm), 75% humidity, and 30°C.
