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Finally, Notch signaling is required to differentiate the socket from the shaft cell and the thecogen from the neuron.
This suggests that the slight decrease in Myc protein level observed in the shaft cell in B52 mutant clones is not due only to a developmental delay.
It should be noted that B52 expression is the highest in the macrochaete shaft cell, which may explain why this cell is the most sensitive to B52 overexpression.
Just after pIIa division, Myc is found in both daughter cells (the socket and the shaft) and later accumulates in the shaft cell at 22 24 hr APF.
Together, our results show that Brat overexpression counteracts the effect of B52 on myc expression and growth and rescues the shaft cell death induced by B52 overexpression.
The absence of macrochaete bristles in SOP > B52 adults is partially rescued by co-overexpression of the caspase inhibitor P35, indicating that the shaft cell dies through apoptosis.
Similar(51)
This was confirmed by immuno-detection (frame 9) showing that the left cluster was formed by four outer cells: two Su(H) positive socket cells (yellow cells) and two shaft cells (green sibling cells), showing that the organ originated from two pIIa cells.
This totals 49 shaft cells, 26 in the clones (B52 −/−), and 23 outside (control, B52 +/−).
Each organ is composed of two outer cells, the socket and shaft cells, and two inner cells, the neuron and sheath cell.
The mean nuclear area is increased upon B52 overexpression by 12% in neuron and sheath cells, by 19% in shaft cells, and by 24% in socket cells.
Comparison of Myc staining between shaft cells, within and outside the clones, reveals a 35% decrease of Myc level in B52 –/– cells.
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