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The odd diploid number in the males was always coupled with the presence of a multiple X1X2Y sex system.
Surprisingly, the F2 progenies had sex ratios very similar to the expected value of a female heterogametic sex system.
Birds have evolved a female heterogametic sex system (male ZZ, female ZW), through stepwise suppression of recombination between chrZ and chrW.
Based only on classical cytogenetic data, it was previously proposed that an initial centric fusion gave rise to the X1X1X2X2/X1X2Y sex system found in karyomorphs B, C and D of E. erythrinus.
It is probable that the same centric fusion also gave rise to the X1X2Y sex system in karyomorphs B and C, since it appears to have originated before the divergence of these three karyomorphs [ 34] (Fig. 4).
Complex epistatic sex system at fishes has been found consisting of a major female heterogametic ZW locus on chromosome 5, two separate male heterogametic XY loci on chromosome 7, and two additional interacting loci on chromosomes 3 and 20 [ 9].
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Nonetheless, XY sex systems are not common in fish.
The results characterized the chromosomes that gave rise to the multiple sex determination systems and that both sex systems originated from different autosomal pairs.
The Erythrinidae fish family is characterized by a large variation with respect to diploid chromosome numbers and sex-determining systems among its species, including two multiple X1X2Y sex systems in Hoplias malabaricus and Erythrinus erythrinus.
Different autosomes were first converted into a poorly differentiated XY sex pair in each species, and additional chromosomal rearrangements produced both X1X2Y sex systems that are currently present.
We used reciprocal cross-species FISH with X and Y probes to analyze the origin of the sex systems in these two genera.
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