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Similarly to TSA, HC-toxin reduced the severity of wing defects (Fig. S1, Table S1).
The breadth and severity of wing patterning defects associated with Gro overexpression demonstrate the pleiotropic nature of Gro in development and the sensitivity of the gene regulatory network to the concentration of functional Gro in the nucleus.
Sex differences in the severity of wing phenotypes were minimal.
Conditions that increased the probability of observing wings with vesicles also increased the severity of wing defects when they occurred.
The severity of wing phenotypes also varied across different genetic backgrounds, and expressivity was positively correlated with penetrance.
We examined flies with vs containing X chromosomes and quantified the severity of wing phenotypes for both sexes, four different autosomal backgrounds, and four different developmental temperatures.
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When expressed in wing discs, these RNAi lines caused wing phenotypes indicative of Dpp or Gbb inactivation, and the severity of the wing phenotypes indicated that UAS-Dpp-RNAi S and UAS-Gbb-RNAi S are strong lines whereas UAS-Dpp-RNAi W and UAS-Gbb-RNAi W are weak lines.
With increasing concentrations of TSA in the food during larval development, we observed decreasing severity of the wing blistering.
This deletion derivative of the P[lacW]k06808 insertion also behaves as a recessive mutation, with the only difference between pkn dlnΔ5 and the original pkn dln allele being a reduction in the severity of the wing phenotype (Table 2).
The apparent subtlety of pathology seen with the naked eye belies the prevalence, severity and extent of wing damage in WNS, and is likely to be one of the reasons for an underappreciation of G. destructans as a primary pathogen.
The level of Ubx upregulation correlates with the severity of the adult wing phenotypes observed [34].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com