We found that the size of the duplication did not correlate with the severity of the phenotype in several cases.
Although the severity of the phenotype defects varied somewhat, two typical severe defect phenotypes, "half pupation" larvae and "abnormal eclosion" adults, were observed (Fig. 4B, C, H, I).
In the presence of just one copy of wild-type Rpd3, the average severity of the phenotype was much reduced – 31% showed severe blistering, 10% moderate blistering, 24% mild blistering, and 34% no blistering (Fig. 5B, purple bars).
The severity of the phenotype was correlated with the level of QHB expression.
A correlation was observed between the accumulation of siRNA and severity of the phenotype.
The severity of the phenotype was positively correlated with Cg1 expression level.
These usually consist of large genomic deletions, the extents of which are not correlated with the severity of the phenotype.
However, as the Rab10 mutation in mice was difficult to study due to the severity of the phenotype, conditional knockout strategy is required for further clarification of Rab10's function.
In all cases, the transgenic lines expressing the mutant gamma subunit behave in the same way as the α- and/or the β-deficient mutants, albeit with reduced severity of the phenotype.
The severity of the phenotype correlated with both increased STGalT mRNA and protein levels but no differences were observed between N-glycosylation profiles of plants with and without the phenotype.
This may explain the severity of the phenotype.
