Response surface methodology was applied to analyze results based on the combined severity of pretreatment experiments.
The sustained hunger neuropeptide profile during restriction, combined with the data on extent and severity of restriction (experiments one and two) on the PRH response suggested that a peripherally generated signal, produced either by the depleted fat or lean tissue, was stimulating appetite and generating the PRH (Dulloo et al., 1997).
This included topics related to ethics, legislation, animal housing, breeding and handling, animal behaviour, health monitoring, evaluation of categories of severity of an experiment, recognition of and methods to reduce pain, distress and other unhealthy conditions, reporting and common experimental techniques.
Unlike young age [11], compromised periodontal condition was not associated with the severity of changes in animal experiments [12].
These differences in the severity of take-all in Experiment 3 were associated with differences in thousand-grain weights (35.8 and 34.7 g, respectively) but not in total yields of grain.
In Experiment 9, mean yield of grain was only 6.29 t ha−1, reflecting the greater severity of take-all than in Experiment 8, and there was a very significant (P < 0.001)negative linear effect of population density.
These procedures were later modified by Willner resulting in higher length of experiment and lower severity of stressor [ 16].
We describe experiments correlating the severity of the phenotypes with P450 SU1 expression levels and not AtSUC2 expression levels, and report on further experiments indicating that CYP105A1 from S. griseolus disrupts brassinosteroid homeostasis in these transgenic plants.
Walters et al. showed that the orally bioavailable CCR9 antagonist CCX282-B can ameliorate, in one single experiment, the severity of small intestinal chronic inflammation of TNFΔare mice [29].
The animals were evaluated every two to three days for frequency and severity of arthritis until termination of the experiments.
