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(G ) Final (32 hAPF) measured area of unperturbed and the indicated laser severed wing blades.
The proximally severed wing undergoes significant but short-lived PD shear during the molting process until 18 hAPF.
In contrast, the offset is zero for the proximally severed wing blade, which does not shrink immediately after ablation.
Left corner inset in (A ) shows the severed wing at 32 hAPF, with the tracked region highlighted in green.
(A - C ) Show total shear rate (A ) and total accumulated shear (B ), along with their cellular contributions, in a distally severed wing blade (dist-sev#2).
Since the analysis of dumpy ov1 and proximally severed wing shows that PD T1 transitions are stress dependent, this suggests that there is a time delay between the change in tissue stress and the resulting T1 transitions.
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We expected that cellular events that depended on externally generated stresses would be even more strongly perturbed in proximally severed wings than in dumpy ov1 or distally-severed wings.
The severed wings are glued onto the base of a shaker, which is capable of inducing translational motion in the lateral direction of the wing plane.
We spent hours wiping dead ladybug guts out of the bottoms of shoes, picking severed wings out of sequined jackets, and, in a last ditch effort to save a fancy piece of clothing we didn't own, stuck a chiffon dress in our office freezer for two days to get rid of all the stubborn bastards that crawled into its layers.
Furthermore, like dumpy ov1 and distally severed wings, proximally severed wings undergo greater AP shear resulting from correlation effects.
Consistent with this, the final shape of distally severed wings resembles that of dumpy ov1 wings.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com