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The main challenge in cultivating microorganisms producing biosurfactants is to overcome this severe foam production.
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The current study introduces an alternative to produce a biosurfactant that overcomes the challenges of severe foaming and need for additional constructions.
In 'wet' digestion a fine particle size led to severe foaming and the process could not be operated above 5 kg VS m−3 day−1.
Bacillus subtilis ATCC 21332 was used to produce a lipopeptide-type biosurfactant (namely, surfactin) in an innovative bioreactor tailored to solve the problems of severe foaming arising from production of the biosurfactant.
As cell lysis with hypochlorite is strongly exergonic and generates a severe amount of foam, in- or external cooling was applied, depending on the total volume of the vessel to prevent a strong temperature increase.
In the phlebology field, reversible cardiac arrest [ 18] and transient neurological defects [ 19, 20] are rare but severe complications associated with foam sclerosants because of overdose and subsequent leakage to the systemic veins.
The steps used to prepare the seed cultivation and medium components were the same as described in the previous section, except for the addition of 0.05 % antifoaming agent in the fermentation medium, which prevented foaming under severe agitation.
By using fully allogeneic arterial grafts transplanted into normolipidemic wild-type or hyperlipidemic ApoE−/− mice we show that the hyperlipidemic recipients develop severe TA, characterized by intense foam cell infiltration into the neointima.
The seizures grew more severe and, by his mid-teens, he would foam at the mouth and bite his tongue, while his limbs convulsed rhythmically.
Many protesters have severe reactions to the gas, including seizures and foaming at the mouth.
As Dr Armaiti May, a veterinary expert, testified during my trial, some of the pigs were foaming at the mouth and in "severe distress", appearing to breathe as quickly as 180 breaths per minute.
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