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This may either be explained by functional redundancy or the need to repress several cell wall functions and/or components in order to cause severe cell wall damage.
Microscopical and ultrastructural observations revealed a severe cell wall defect of pun1∆ mutants.
The corresponding deletant strains have severe cell wall defects that may explain their high basal Slt2 activation.
Second, their assays were all performed in a strain lacking both wsc1 ∆ and mid2 ∆, and therefore the strain had a more severe cell wall defect.
It is considered as crucial structural cell wall component since in baker's yeast the lack of CCW12 results in severe cell wall damage and reduced mating efficiency.
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At the indicated time points, the turbidity of each sample was measured immediately after the addition of 100 µl aliquot from the reaction tube into a spectrophotometer cuvette already containing 800 µl media and 100 µl of 10% SDS (final conc. 1%), which rapidly lyse cells with severe cell-wall damages.
Water loss in this matrix results in a severe disruption of cell wall integrity, which can be irreversible [ 33].
In the production of bioethanol from lignocellulosic biofuel crops, one of the principal energy inputs arises from the need for a severe pretreatment of the cell wall matrix prior to enzymatic saccharification to increase access to the structural sugar polymers [ 1].
Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants.
lrx1 lrx2 double mutants show a severe defect in root hair cell wall structures and growth, suggesting a role of LRX1 and LRX2 in cell wall formation [ 24, 26].
With IL-17R-deficient bone marrow chimeric mice, it was reported that the development of severe destructive streptococcal cell wall-induced arthritis was particularly dependent on the presence of intact signalling in radiation-resistant cells [ 133].
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