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During the compression from ambient to 2 GPa at room temperature, the samples' peaks broaden asymmetrically, with a much more severe broadening on the large angular dispersive (2θ) side of the peak, as shown in the bottom two curves of Fig. 1.
For example, G2496 and G2497 are mobile in the isolated dimerisation domain but become immobilised in talin 2294 2541, as shown by severe broadening of the resonances.
Characterisation of the structure and dynamics of large biomolecules and biomolecular complexes by NMR spectroscopy is hampered by increasing overlap and severe broadening of NMR signals.
The formation of a large molecular assembly from the facially amphiphilic dendron would cause severe broadening and attenuation of the F signal as shown in Figure 1.
Inserting an equivalent amount of water into the reference path, e. g. for dispersion compensation, would lead to a more severe broadening of the PSF.
The elongated coiled-coil structure formed by self-association may render Cby to tumble significantly slower than a globular protein with similar molecular weight, resulting in severe broadening of the NMR peaks from this portion of the protein.
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The equilibrium between the two tautomers causes severe line broadening of the His22 imidazole ring nitrogen resonances in the wild-type protein, with less severe line broadening for the P23T HgD and the P23S and P23V mutants.
It can be observed from this figure that less severe spectral broadening is observed for the DF relaying option.
Due to severe line broadening at 15°C, the RNA molar ratio could only reach 1.6 for precise chemical shift measurements.
Note the NMR experiment could not be performed at 10°C due to severe line broadening of the free protein.
For the decode-and-forward (DF) relaying option, it is observed that less severe spectral broadening are observed.
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