Although each study has resulted in several unique cross-linked peptides, it is noted that many of the identified interlinked lysines are located in very close proximity within the sequence of cytochrome C. For example, while K53 to K79 (11.6 Å) linkage was found with DSSO cross-linking, K55 to K73 (11.6 Å) was only identified by DMDSSO cross-linking.
Among the zebra finch peptides discovered were several unique vasoactive intestinal and adenylate cyclase activating polypeptide 1 peptides created by cleavage at sites previously unreported in mammalian prohormones.
We show that several standard benchmark datasets of unique peptides used in such studies contain a substantial number of peptides that share a high degree of sequence identity with one or more other peptide sequences in the same dataset.
Compared to the traditional antibiotics, cationic antimicrobial peptides (AMPs) exhibit several unique characteristics, including the ability to rapidly kill target cells, broad spectrum activity against serious antibiotic-resistant pathogens in the clinic, and the relative difficulty in selecting resistant mutants in vitro (Jenssen et al., 2006; Huang et al., 2010b).
Five unique peptides were identified matching several members of the α-actinin family (actinin1, 2, 3, 4); nevertheless only one member of the family, α-actinin 1, aligned to each of the five peptides with 100% identity.
In addition, several unique NH2- and COOH-terminal truncated Aβ peptides were observed in the AD brain lysates.
The alignment data (Additional file 1: Figure S27) showed several unique features that were not observed in the alignments to type 1 and type 3 peptides.
An additional 15 isoforms lacked one or more unique peptides to distinguish them from other isoforms or from each other, but several of these must be expressed.
Several studies [5], [15] [17], [19] have reported the performance of MHC-II binding peptide prediction methods using datasets of unique peptides.
