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Second, each genomic locus may generate several types of transcripts, either because of genomic variants (i.e. there may exist several alleles for a locus) or because of transcriptomic variants (i.e. alternative splicing or alternative transcription start/end may generate several transcripts from a single locus that differ by the inclusion or exclusion of subsequences).
Most genes are subjected to alternative splicing following transcription resulting in the production of several transcripts from each gene [ 10, 11].
Although care is needed when inferring function based on a list like this, it is notable that among the top rated DE genes in both generations were several transcripts from immune genes, which are separately shown in Table 3.
Alternative splicing is an important process in higher eukaryotes that allows obtaining several transcripts from one gene.
In all exoribonuclease mutants there are also several transcripts from the anaerobic respiration functional category which were considerably affected.
Although the whole transcriptome is much smaller than the whole genome, in the context of structural alterations, RNA-Seq data can be more difficult to analyze, partially due to splicing, which can produce several transcripts from the same gene.
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During the last stage of acorn development (S8, cluster F) several transcripts protrude from the list of DEGs annotated as related to water responses.
Several transcripts derived from Fusarium spp. with predicted involvement in amino acid salvage and recycling of nitrogenous waste products were detected.
Altogether, our data reveal that several transcripts expressed from the sRkB and sRkC loci have K-turn motifs that might be important for their cellular function.
A recent nuclear transcriptome analysis of dinotoms, despite using dinoflagellate-specific primers, identified several transcripts probably originating from the endosymbiont nucleus, implying a significant amount of DNA being transcribed in this nucleus (Burki et al. 2014).
In cases where several transcripts of different lengths from the same gene were found, supposedly representing different splicing variants, only the longest sequence was retained.
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